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Animal cloning (Dolly the sheep): uses in research

Also: Dolly, somatic cell nuclear transfer, SCNT, cloned mammals

Cloning by nuclear transfer puts the nucleus of a body cell into an egg cell. Dolly the sheep (born 1996, reported 1997) was the first mammal cloned from an adult cell. Later work cloned mice and monkeys.

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Timeline

  1. 1996 Sheep cloned from cultured cells
  2. 1997 Dolly the sheepPolly: cloned transgenic sheep
  3. 1998 Cloned mice
  4. 2018 Cloned macaque monkeys

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biology

lab biology 1996

Sheep cloned from cultured cells

Campbell and colleagues cloned sheep (Megan and Morag) by nuclear transfer from a cultured cell line.

Built / tested: cloned sheep

As reported in: Campbell, McWhir, Ritchie et al. (1996). Sheep cloned by nuclear transfer from a cultured cell line. Nature. doi:10.1038/380064a0

Abstract only

lab biology 1997

Dolly the sheep

Wilmut and colleagues reported lambs from fetal and adult cells, including Dolly, cloned from an adult mammary cell.

Publisher update notice: correction

Built / tested: Dolly the sheep

As reported in: Wilmut, Schnieke, McWhir et al. (1997). Viable offspring derived from fetal and adult mammalian cells. Nature. doi:10.1038/385810a0

Abstract only

lab biology 1998

Cloned mice

Wakayama and colleagues produced full-term cloned mice from cumulus cell nuclei.

Built / tested: cloned mice

As reported in: Wakayama, Perry, Zuccotti et al. (1998). Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature. doi:10.1038/28615

Abstract only

lab biology 2018

Cloned macaque monkeys

Liu and colleagues cloned macaques (Zhong Zhong and Hua Hua) by somatic cell nuclear transfer.

Publisher update notice: erratum

Built / tested: cloned monkeys

As reported in: Liu, Cai, Wang et al. (2018). Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer. Cell. doi:10.1016/j.cell.2018.01.020

Read free · publisher open access

biomedical

lab biomedical 1997

Polly: cloned transgenic sheep

Schnieke and colleagues produced cloned sheep carrying a human clotting factor IX gene.

Built / tested: transgenic cloned sheep

As reported in: Schnieke, Kind, Ritchie et al. (1997). Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts. Science. doi:10.1126/science.278.5346.2130

Abstract only